Journal: PLoS ONE

Article Title: Phenotypic Variation in the Plant Pathogenic Bacterium Acidovorax citrulli

doi: 10.1371/journal.pone.0073189

Figure Lengend Snippet: Cultures were grown in XVM2 medium for 48 hours in polystyrene ELISA plates at 28 ° C. Formed biofilms at the medium/air interface were stained with 0.01% crystal violet stain. Stained biofilms were resuspended with ethanol and quantitatively estimated by optical density measurements at 595 nm. Top, middle and bottom panels show the results of three independent experiments (first, second, third; with five replicates per strain in the first experiment, and ten replicates per strain in second and third experiments). The box-and-whisker plots indicate minimum, first quartile, median, third quartile, and maximum values. Different letters indicate statistically significant differences (p < 0.05; for details see Appendix S1) among strains in each experiment.

Article Snippet: After 2 hours, 250 μl of the suspensions of each well were collected, transferred to new 96-well plates, and the optical densities of the suspensions were measured at 595 nm using an Infinite F200 ELISA plate reader (Tecan Group Ltd., Männedorf, Switzerland).

Techniques: Enzyme-linked Immunosorbent Assay, Staining, Whisker Assay